Objective: To summarize the clinic experience of flexible ureteroscope in diagnosis and treatment of upper urinary tract disease. Method: From Nov 2013 to Dec 2015, 60 hematuresis cases underwent flexible ureteroscope for diagnosis and treatment of upper urinary tract. Clinical data was analyzed retrospectively. Results: All the cases were diagnosed and cured successfully in a one-time success .19 cases were diagnosed epithelium cancer, 6 cases were diagnosed non-specificity pyeloureteritis, 18 cases were diagnosed as calculus of ureter, and 8 cases were renal pelvis venous hemorrhage, no abnormality seen in 9 cases. No serious complication happened. And the time of the surgeries was 10 similar to 70 min and the average time was 40 min. Hospitalization time was 3 similar to 9 days, and the average time was 4 days. Conclusion: Flexible ureteroscope can be used to diagnose and cure tumor, calculus, inflammation and other diseases of upper urinary tract, especially, to upper urinary minimal lesion. Flexible ureteroscope has an obvious advantage. From Nov 2013 to Dec 2015, 58 hematuria patients underwent flexible ureteroscope for diagnosis and treatment of upper urinary tract diseases. Now we report as followed.