Bacopaside I is one of the main pseudojujubogenin glycosides isolated from Bacopa monniera. In the present study, a rapid and robust LC-ESI-MS/MS method was developed and validated to quantify bacopaside I in rat plasma. After plasma samples were deproteinized by methanol, the post-treatment samples were analyzed on a Zorbax Eclipse Plus C-18 (2.1 x 50 mm, 1.8 mu m) column using a mobile phase of acetonitrile and water (65:35, v/v). Detection was performed on a triple-quadrupole tandem mass spectrometer with selected reaction monitoring (SRM) mode via electrospray ionization source. This method covered a linearity range of 10-2000 ng/mL with the lower limit of quantification of 10 ng/mL. The intra- and inter-day precisions of analysis were less than 10.2%, and the accuracies were between -11.1% and 8.4% at the concentrations of 25, 150 and 1800 ng/mL. The total run time was 6.0 min. This method was successfully applied to the preclinical pharmacokinetic study of bacopaside I following intravenous or oral administration to rats. (C) 2016 Elsevier B.V. All rights reserved.
[1]Jilin Univ, China Japan Union Hosp, Dept Orthoped, Changchun 130033, Jilin, Peoples R China.
[2]Cent S Univ, Sch Basic Med Sci, Dept Anat & Neurobiol, Changsha 410013, Hunan, Peoples R China.
[3]Jilin Univ, Hosp 1, Dept Clin Epidemiol, Changchun 130021, Peoples R China.
[4]Jilin Univ, Hosp 2, Dept Anesthesia, 218 Ziqiang St, Changchun 130021, Peoples R China.
[5]Jilin Univ, Hosp 1, Dept Spine Surg, 71 Xinmin St, Changchun 130021, Peoples R China.
(8.0+极速模式)