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HSP70 signaling in renal tubular epithelial cells injury induced by ischemia 期刊论文

编号：

19038d6e-d0a8-4063-b553-b58c0e726fed
作者：

Yin, Jian(尹健)#^[1]Xu, Ruisi(徐瑞斯)^[2]Wei, Jun^[3];Zhang, Siqi(张斯棋)*^[4]
语种：

英文
期刊：

LIFE SCIENCES ISSN：0024-3205 2019 年 223 卷 (88 - 94) ; APR 15
收录：
关键词：

GRX1 DJ-1 HSP70 OGD HK-2
摘要：

Aims: Gluaredoxin1 (GRX1) is an important protein of the cellular antioxidant defense system, but its role in renal epithelial cell injury caused by ischemia remains unclear. In this study, we aimed to gain insight into the role of GRX1 in HK-2 cells with oxygen glucose deprivation (OGD) injury, which served as an in vitro cell model of renal epithelial cell ischemic injury. We investigated the underlying regulation of GRX1, DJ-1, and HSP70 as well as the role of the GRX1/DJ-1/HSP70 signaling pathway in this model.
Materials and methods: The protein and mRNA expressions were measured by Western blot and qRT-PCR assays, respectively. GRX1 was overexpressed by transfection of pcDNA.3.1-GRX1 and DJ-1 was inhibited by transfection with DJ-1 siRNA. Cell apoptosis, caspase-3 activity, lactate dehydrogenase (LDH) leakage, or superoxide dismutase (SOD) content was tested by the related detection kit. Reactive oxygen species (ROS) level was detected via carboxy-H2DCF-DA.
Key findings: We found that GRX1 was distinctly down-regulated in HK-2 cells after incubation under the OGD condition. GRX1 overexpression markedly constrained cell apoptosis, caspase-3 activity, LDH leakage, and the ROS level, while SOD content was elevated. GRX1 up-regulation increased DJ-1 and HSP70 protein expression, while DJ-1 inhibition significantly offset the effect of GRX1 overexpression on HSP70, indicating that GRX1 could regulate HSP70 via control of DJ-1. Moreover, we observed that HSP70 inhibition removed the constraints imposed by GRX1 overexpression on ROS level, LDH leakage, and caspase-3 activity.
Significance: Overall, this study showed that GRX1 minimizes cell injury and apoptosis in HK-2 cells under OGD conditions via regulation of DJ-1 and HSP70 expression.
推荐引用方式
GB/T 7714：

Yin Jian,Xu Ruisi,Wei Jun, et al. The protective effect of glutaredoxin 1/DJ-1/HSP70 signaling in renal tubular epithelial cells injury induced by ischemia [J].LIFE SCIENCES,2019,223:88-94.
APA：

Yin Jian,Xu Ruisi,Wei Jun,Zhang Siqi.(2019).The protective effect of glutaredoxin 1/DJ-1/HSP70 signaling in renal tubular epithelial cells injury induced by ischemia .LIFE SCIENCES,223:88-94.
MLA：

Yin Jian, et al. "The protective effect of glutaredoxin 1/DJ-1/HSP70 signaling in renal tubular epithelial cells injury induced by ischemia" .LIFE SCIENCES 223(2019):88-94.
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