首页 / 院系成果 / 成果详情页

MiR-27a Promotes Hepatocellular Carcinoma Cell Proliferation Through Suppression of its Target Gene Peroxisome Proliferator-activated Receptor gamma 期刊论文

编号：

1c57dc58-9e01-4227-abf4-ae3c2cb687e8
作者：

Li, Shuo(李硕)#^[1]Li, Jing^[2];Fei, BingYuan(费秉元)^[3]Shao, Dan^[2];Pan, Yue^[2];Mo, ZhanHao(莫展豪)^[4]Sun, BaoZhen(孙宝震)^[1]Zhang, Dan(张丹)^[1]Zheng, Xiao^[2];Zhang, Ming^[2];Zhang, XueWen(张学文)*^[1]Chen, Li^[2];
语种：

英文
期刊：

CHINESE MEDICAL JOURNAL ISSN：0366-6999 2015 年 128 卷 7 期 (941 - 947) ; APR 5
收录：
关键词：

Cell Proliferation Hepatocellular Carcinoma MiR-27a Peroxisome Proliferator-activated Receptor gamma
摘要：

Background: MicroRNAs (miRNAs) function as essential posttranscriptional modulators of gene expression, and are involved in a wide range of physiologic and pathologic states, including cancer. Numerous miRNAs are deregulated in hepatocellular carcinoma (HCC). This study aimed to investigate the role of miR-27a in the development of HCC.
Methods: The expression of MiR-27a was measured by quantitative real-time polymerase chain reaction (qRT-PCR). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide was used to examine changes in the viability of HepG2, Bel-7402, Bel-7404 hepatoma cell lines associated with up-regulation or down-regulation of miR-27a. A dual-luciferase activity assay was used to verify a target gene of miR-27a. Immunohistochemistry, qRT-PCR, Western blotting analysis, and cell cycle and apoptosis flow cytometric assays were used to elucidate the mechanism by which miR-27a modulates liver cancer cell proliferation.
Results: The expression of miR-27a was signi.cantly increased in HCC tissues and HepG2, Bel-7402, Bel-7404 hepatoma cell lines (P < 0.05). We also found that the down-regulation of miR-27a in HepG2 cells dramatically inhibited proliferation, blocked the G1 to S cell cycle transition and induced apoptosis (P < 0.05). In addition, miR-27a directly targeted the 3"-untranslated region of peroxisome proliferator-activated receptor gamma (PPAR-gamma), and ectopic miR-27a expression suppressed PPAR-gamma expression on the mRNA and protein levels. The rosiglitazone-induced overexpression of PPAR-gamma attenuated the effect of miR-27a in HCC cells.
Conclusions: Our findings suggested that miRNA-27a promoted HCC cell proliferation by regulating PPAR-. expression. MiR-27a may provide a potential therapeutic strategy for HCC treatment.
推荐引用方式
GB/T 7714：

Li Shuo,Li Jing,Fei Bing-Yuan, et al. MiR-27a Promotes Hepatocellular Carcinoma Cell Proliferation Through Suppression of its Target Gene Peroxisome Proliferator-activated Receptor gamma [J].CHINESE MEDICAL JOURNAL,2015,128(7):941-947.
APA：

Li Shuo,Li Jing,Fei Bing-Yuan,Shao Dan,&Chen Li.(2015).MiR-27a Promotes Hepatocellular Carcinoma Cell Proliferation Through Suppression of its Target Gene Peroxisome Proliferator-activated Receptor gamma .CHINESE MEDICAL JOURNAL,128(7):941-947.
MLA：

Li Shuo, et al. "MiR-27a Promotes Hepatocellular Carcinoma Cell Proliferation Through Suppression of its Target Gene Peroxisome Proliferator-activated Receptor gamma" .CHINESE MEDICAL JOURNAL 128,7(2015):941-947.
条目包含文件：

文件类型：PDF,文件大小：

正在加载全文

未找到全文

浏览次数：105  下载次数：0

分享到：

浏览次数：105

下载次数：0

打印次数：0