Protein biomarkers are very important indicators of diseases and have great potential in cancer early diagnosis. The majority of detection methods for protein biomarkers currently rely on specific capture antibodies or aptamers with chemiluminescent and fluorescent labels. Here, an antibody-free strategy for discrimination of versatile proteins is proposed based on surface- enhanced Raman spectroscopy. The SERS spectral variation of a Symmetry Breaking linker molecule, perylenetetra carboxylic acid (PTCA), is found to Frequency Shin directly correlate with the protein types, according to which protein biomarkers even homologous proteins with very similar molecular structures can be discriminated with the aid of hierarchical cluster analysis. Furthermore, the feasibility of the proposed approach has been proved in early liver cancer diagnosis with clinical samples. All the results indicate that PTCA as a universal SERS probe has great potential in rapid, accurate, and direct protein biomarker discrimination in cancer diagnosis.
[1]Jilin Univ, State Key Lab Supramol Struct & Mat, Changchun 130012, Jilin, Peoples R China.
[2]Jilin Univ, China Japan Union Hosp, Changchun 130033, Jilin, Peoples R China.
[3]Jilin Normal Univ, Minist Educ, Key Lab Preparat & Applicat Environm Friendly Mat, Changchun 130103, Jilin, Peoples R China.
[4]Univ Manchester, Sch Biol Sci, Fac Biol Med & Hlth, Manchester M13 9PT, Lancs, England.
(8.0+极速模式)