Glial fibrillary acidic protein (GFAP) is one of the best markers for the activation of astrocytes (AS) following injury or stress in the central nervous system (CNS). The purpose of this study was to examine the expression of GFAP and 14-3-3 epsilon in rat AS subjected to hypoxia. We established primary cultures of AS from cerebral cortex of neonatal Sprague-Dawley rats as a model of glucose deficiency and hypoxia/ischemia-reperfusion. We analyzed the activated astrocyte markers GFAP and 14-3-3e by western blot analysis and found that both increased over time, starting at 4 h and reaching the highest level at 72 h, at the end of the experiment. GFAP and 14-3-3 epsilon protein localization by double-labeling immunofluorescence showed elevated expression and co-localization in the cytoplasm of AS. GFAP and 14-3-3 epsilon expression remained elevated in AS 72 h after stress conditions, which is possibly related to the excessive activation and dysfunction of the CNS in chronic injuries.
[1]Tianjing Nankai Hosp, Dept Orthoped, Tianjin 300100, Peoples R China.
[2]Jilin Univ, Hosp 2, Dept Orthoped, Changchun 130041, Jilin, Peoples R China.
[3]Jilin Univ, China Japan Union Hosp, Dept Med, Changchun 130033, Jilin, Peoples R China.
[4]Jilin Univ, China Japan Union Hosp, Dept Orthoped, 126 Xiantai St, Changchun 130033, Jilin, Peoples R China.
(8.0+极速模式)