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Knockdown of Long Non-Coding RNA RP11-445H22.4 Alleviates LPS-Induced Injuries by Regulation of MiR-301a in Osteoarthritis 期刊论文

编号：

294fcf9d-f4e2-4b7b-b8e6-fc024370b135
作者：

Sun, Taitao#^[1]Yu, Jian#^[2]Han, Liang^[3];Tian, Shuo^[4];Xu, Bin^[1];Gong, Xianbin^[1];Zhao, Qiang(赵强)^[1]Wang, Yang(王杨)*^[5]
语种：

英文
期刊：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY ISSN：1015-8987 2018 年 45 卷 2 期 (832 - 843) ; 2018
收录：
关键词：

LncRNA RP11-445H22.4 MiR-301a Cell viability Apoptosis Inflammatory injury CXCR4
摘要：

Background/Aims: Several long non-coding RNAs (lncRNAs) play vital roles in osteoarthritis (OA), whereas the role of lncRNA RP11-445H22.4 in OA remains unclear. The study aimed to investigate the effect of lncRNA RP11-445H22.4 on lipopolysaccharide (LPS)-induced cell viability, apoptosis and inflammatory injury of OA. Methods: The expression of RP11-445H22.4, miR-301a and CXCR4 in human cartilage ATDC5 cells were altered by transfection, and then cells were exposed to 5 mu g/ml LPS for 12 h. Then cell viability, apoptosis, apoptosis-related factors and inflammatory cytokines were analyzed by CCK-8, flow cytometry, western blot, RT-qPCR and ELISA, respectively. Dual-luciferase reporter assay was performed to assess the binging sites of RP11-445H22.4 and miR-301a. The signal pathways of NF-kappa B and MAPK/ERK were determined by western blot. Results: LPS reduced cell viability, increased apoptosis and stimulated release of IL-1 beta, IL-6, IL-8 and TNF-alpha. However, RP11-445H22.4 inhibition significantly rescued LPS-induced injuries by promoting cell viability, suppressing apoptosis and inflammatory cytokines secretions in ATDC5 cells. In addition, miR-301a directly bound to RP11-445H22.4, and suppression of miR-301a inversed the effects of RP11-445H22.4 inhibition. Furthermore, CXCR4 was a direct target of miR-301a, and CXCR4 silencing increased cell viability, decreased apoptosis and inflammatory cytokines secretions in LPS-treated ATDC5 cells. Besides, we found that CXCR4 silencing blocked LPS-activated NF-kappa B and MAPK/ERK pathways. Conclusions: The study indicated that lncRNA RP11-445H22.4-miR-301a-CXCR4 axis played an important role in cartilage ATDC5 cells and provided a theoretical basis of lncRNA RP11-445H22.4 in OA. (C) 2018 The Author(s) Published by S. Karger AG, Basel
推荐引用方式
GB/T 7714：

Sun Taitao,Yu Jian,Han Liang, et al. Knockdown of Long Non-Coding RNA RP11-445H22.4 Alleviates LPS-Induced Injuries by Regulation of MiR-301a in Osteoarthritis [J].CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2018,45(2):832-843.
APA：

Sun Taitao,Yu Jian,Han Liang,Tian Shuo,&Wang Yang.(2018).Knockdown of Long Non-Coding RNA RP11-445H22.4 Alleviates LPS-Induced Injuries by Regulation of MiR-301a in Osteoarthritis .CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,45(2):832-843.
MLA：

Sun Taitao, et al. "Knockdown of Long Non-Coding RNA RP11-445H22.4 Alleviates LPS-Induced Injuries by Regulation of MiR-301a in Osteoarthritis" .CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 45,2(2018):832-843.
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