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Let-7g regulates p16ink4a/p19arf and inhibits proliferation of glioma cells: roles of HMGA2 期刊论文

编号：

2bce7b98-1927-445d-bdcf-f1eb79800677
作者：

Guo, Baofeng(郭宝锋)#^[1]Liu, Zhenwen^[2];Ma, Hongxi^[3];Yun, Wenjing^[2];Zhang, Ling(张灵)*^[2]
语种：

英文
期刊：

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY ISSN：1936-2625 2016 年 9 卷 4 期 (4601 - 4614)
收录：
关键词：

Let-7g HMGA2 p16ink4a p19arf glioma proliferation apoptosis xenograft model
摘要：

We carried out this study for the purpose of unveiling the association among let-7g, HMGA2 and p16ink4a/p19arf pathway in glioma. This study incorporated 78 glioma tissues which were collected from glioma patients and 10 normal brain tissues. Both real-time quantitative RT-PCR (qRT-PCR) and in situ hybridization were implemented for the detection of let-7g expression level in tissues and cells. Moreover, immunohistochemistry and western blot assay were used to measure HMGA2, p16ink4a and p19arf. We also transfected different groups of vectors into NGA cells and these vectors include let-7g inhibitors, lenti-HMGA2 and let-7g inhibitors + HMGA2 siRNA. U251 cells also were transfected with different groups of vectors including let-7g mimics, HMGA2 siRNA and let-7g mimics + lenti-HMGA2. Cell proliferation and apoptosis status were assessed by standard assays. Glioma xenograft models were constructed on mice in order to unfold effects of let-7g and HMGA2 on glioma tissues in vivo. Let-7g expression was significantly down-regulated in both glioma tissues and cells, while HMGA2 was significantly up-regulated compared to the reference group of normal brain cells (all P < 0.05). As demonstrated by transfected NGA cells, both let-7g inhibitors and lenti-HMGA2 significantly increased HMGA2 expression, decreased both ink4a and arf expressions and up-regulated the proliferation rate of glioma cells, while HMGA2 siRNA antagonized the effect of let-7g inhibitors on glioma cells (all P < 0.05). Transfected U251 cells suggested that let-7g mimics and HMGA2 siRNA significantly decreased HMGA2 expression, increased both ink4a and arf expressions, down-regulated the proliferation rate of glioma cells and up-regulated the apoptosis rate of glioma cells, whereas lenti-HMGA2 antagonized the effect of let-7g mimics on glioma cells (all P < 0.05). As suggested by glioma xenograft models, let-7g mimics and HMGA2 siRNA effectively inhibited the growth of glioma tumors. Let-7g may have significant impact on the proliferation and apoptosis of glioma cells via mediating p16ink4a/p19arf by targeting HMGA2.
推荐引用方式
GB/T 7714：

Guo Baofeng,Liu Zhenwen,Ma Hongxi, et al. Let-7g regulates p16ink4a/p19arf and inhibits proliferation of glioma cells: roles of HMGA2 [J].INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,2016,9(4):4601-4614.
APA：

Guo Baofeng,Liu Zhenwen,Ma Hongxi,Yun Wenjing,&Zhang Ling.(2016).Let-7g regulates p16ink4a/p19arf and inhibits proliferation of glioma cells: roles of HMGA2 .INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,9(4):4601-4614.
MLA：

Guo Baofeng, et al. "Let-7g regulates p16ink4a/p19arf and inhibits proliferation of glioma cells: roles of HMGA2" .INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 9,4(2016):4601-4614.
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