首页 / 院系成果 / 成果详情页

miR-217 inhibits proliferation, migration, and invasion via targeting AKT3 in thyroid cancer 期刊论文

编号：

3043b2ff-bccd-443b-9791-3f1294d5219a
作者：

Lin, Yuanqiang(林元强)#^[1]Cheng, Kailiang(程凯亮)#^[2]Wang, Tongtong(王桐桐)^[3]Xie, Qian^[3];Chen, Minglong(陈明龙)^[3]Chen, Qianqian(陈倩倩)*^[4]Wen, Qiang(温强)*^[3]
语种：

英文
期刊：

BIOMEDICINE & PHARMACOTHERAPY ISSN：0753-3322 2017 年 95 卷 (1718 - 1724) ; NOV
收录：
关键词：

Thyroid cancer miR-217 AKT3 Proliferation Invasion
摘要：

Purpose: The aims of this study were to test the influence of miR-217 on the proliferation, invasion, migration of thyroid cancer and the relevant mechanism.
Method: miR-217 expression levels in thyroid cancer tissues and cell lines were detected by quantitative real-time PCR (qRT-PCR). Cell Counting Kit-8, flow cytometer, wound healing, transwell invasion assays were applied to evaluate the effect of miR-217 on proliferation, apoptosis, migration and invasion of thyroid cells. The luciferase reporter assay, qRT-PCR, and western blot were used to identify target of miR-217. Relative relationship of expression level between miR-217 and AKT3 was analyzed in thyroid cancer tissues. Xenograft transplantation was performed to test effect of miR-217 in vivo.
Results: We found that the expression of miR-217 was significantly decreased in thyroid cancer tissues cell lines. Significantly, decreased miR-217 expression were associated with the clinical stage and lymph node metastasis. Function studies revealed that miR-217 overexpression in thyroid cancer cells inhibited proliferation, migration, and invasion in vitro, as well as suppressed tumor growth in vivo. Subsequently, AKT3 was identified as a target of miR-217 in thyroid cancer. AKT3 expression was upregulated in thyroid cancer tissues, was inversely correlated with miR-217expression. Besides, overexpression of AKT3 efficiently abrogates suppressive effect on proliferation, migration and invasion in thyroid cancer cells caused by overexpression of miR-217.
Conclusion: These data demonstrated a tumor suppressor role for miR-217 in thyroid cancer development and progression by targeting AKT3, suggesting miR-217 might be a potential target for thyroid cancer.
推荐引用方式
GB/T 7714：

Lin Yuanqiang,Cheng Kailiang,Wang Tongtong, et al. miR-217 inhibits proliferation, migration, and invasion via targeting AKT3 in thyroid cancer [J].BIOMEDICINE & PHARMACOTHERAPY,2017,95:1718-1724.
APA：

Lin Yuanqiang,Cheng Kailiang,Wang Tongtong,Xie Qian,&Wen Qiang.(2017).miR-217 inhibits proliferation, migration, and invasion via targeting AKT3 in thyroid cancer .BIOMEDICINE & PHARMACOTHERAPY,95:1718-1724.
MLA：

Lin Yuanqiang, et al. "miR-217 inhibits proliferation, migration, and invasion via targeting AKT3 in thyroid cancer" .BIOMEDICINE & PHARMACOTHERAPY 95(2017):1718-1724.
条目包含文件：

文件类型：PDF,文件大小：

正在加载全文

未找到全文

浏览次数：27  下载次数：0

分享到：

浏览次数：27

下载次数：0

打印次数：0