In this study, we recruited 10 neuromyelitis optica patients, two multiple sclerosis patients and two myelitis patients. Chinese hamster lung fibroblast (V79) cells transfected with a human aquaporin-4-mCherry fusion protein gene were used to detect anti-aquaporin-4 antibody in neuromyelitis optica patient sera by immunofluorescence. Anti-aquaporin-4 autoantibody was stably detected by immunofluorescence in neuromyelitis optica patient sera exclusively. The sensitivity of the assay for neuromyelitis optica was 90% and the specificity for neuromyelitis optica was 100%. The anti-aquaporin-4 antibody titers in sera were tested with serial dilutions until the signal disappeared. A positive correlation was detected between Expanded Disability Status Scale scores and serum anti-aquaporin-4 antibody titers. The anti-aquaporin-4 antibody assay is highly sensitive and specific in the sera of Chinese neuromyelitis optica patients. Detection of aquaporin-4 autoantibody is important for the diagnosis and treatment of neuromyelitis optica.
[1]NE Normal Univ, Membrane Channel Res Lab, Changchun 130024, Jilin, Peoples R China.
[2]Jilin Univ, China Japan Union Hosp, Dept Neurosurg, Changchun 130033, Jilin, Peoples R China.
[3]Jilin Univ, Coll Life Sci, Natl Engn Lab AIDS Vaccine, Changchun 130012, Jilin, Peoples R China.
[4]Univ Bari, Dept Gen & Environm Physiol, I-70126 Bari, Italy.
[5]Univ Bari, Ctr Excellence Comparat Genom CEGBA, I-70126 Bari, Italy.
(8.0+极速模式)