首页 / 院系成果 / 成果详情页

Claudin-15 overexpression inhibits proliferation and promotes apoptosis of Schwann cells in vitro 期刊论文

编号：

5f47875c-c8a4-4e50-a177-fe1109a4bc1e
作者：

Li, JianNan#^[1]Zhang, Zhan(张展)^[1]Wu, GuangZhi(吴广智)^[1]Yao, DengBing^[2];Cui, ShuSen(崔树森)*^[1]
语种：

英文
期刊：

NEURAL REGENERATION RESEARCH ISSN：1673-5374 2020 年 15 卷 1 期 (169 - 177) ; JAN
收录：
关键词：

apoptosis Bax cell proliferation c-Jun Claudin-15 nerve regeneration peripheral nerve injury protein kinase C alpha Schwann cells Wallerian degeneration
摘要：

Our previous experiments have discovered that Claudin-15 was up-regulated in Schwann cells of the distal nerve stumps of rat models of sciatic nerve injury. However, how Claudin-15 affects Schwann cell function is still unknown. This study aimed to identify the effects of Claudin-15 on proliferation and apoptosis of Schwann cells cultured in vitro and explore the underlying mechanisms. Primary Schwann cells were obtained from rats. Claudin-15 in Schwann cells was knocked down using siRNA (siRNA-1 group) compared with the negative control siRNA transfection group (negative control group). Claudin-15 in Schwann cells was overexpressed using pGV230-Claudin-15 plasmid (pGV230-Claudin-15 group). The pGV230 transfection group (pGV230 group) acted as the control of the pGV230-Claudin-15 group. Cell proliferation was analyzed with EdU assay. Cell apoptosis was analyzed with flow cytometric analysis. Cell migration was analyzed with Transwell inserts. The mRNA and protein expressions were analyzed with quantitative polymerase chain reaction assay and western blot assay. The results showed that compared with the negative control group, cell proliferation rate was up-regulated; p-AKT/AKT ratio, apoptotic rate, p-c-Jun/c-Jun ratio, mRNA expression of protein kinase C alpha, Bcl-2 and Bax were down-regulated; and mRNA expression of neurotrophins basic fibroblast growth factor and neurotrophin-3 were increased in the siRNA-1 group. No significant difference was found in cell migration between the negative control and siRNA-1 groups. Compared with the pGV230 group, the cell proliferation rate was down-regulated; apoptotic rate, p-c-Jun/c-Jun ratio and c-Fos protein expression increased; mRNA expression of protein kinase C alpha and Bax decreased; and mRNA expressions of neurotrophins basic fibroblast growth factor and neurotrophin-3 were up-regulated in the pGV230-Claudin-15 group. The above results demonstrated that overexpression of Claudin-15 inhibited Schwann cell proliferation and promoted Schwann cell apoptosis in vitro. Silencing of Claudin-15 had the reverse effect and provided neuroprotective effect.
推荐引用方式
GB/T 7714：

Li Jian-Nan,Zhang Zhan,Wu Guang-Zhi, et al. Claudin-15 overexpression inhibits proliferation and promotes apoptosis of Schwann cells in vitro [J].NEURAL REGENERATION RESEARCH,2020,15(1):169-177.
APA：

Li Jian-Nan,Zhang Zhan,Wu Guang-Zhi,Yao Deng-Bing,&Cui Shu-Sen.(2020).Claudin-15 overexpression inhibits proliferation and promotes apoptosis of Schwann cells in vitro .NEURAL REGENERATION RESEARCH,15(1):169-177.
MLA：

Li Jian-Nan, et al. "Claudin-15 overexpression inhibits proliferation and promotes apoptosis of Schwann cells in vitro" .NEURAL REGENERATION RESEARCH 15,1(2020):169-177.
条目包含文件：

文件类型：PDF,文件大小：

正在加载全文

未找到全文

浏览次数：254  下载次数：0

分享到：

浏览次数：254

下载次数：0

打印次数：0