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MiR-548c impairs migration and invasion of endometrial and ovarian cancer cells via downregulation of Twist 期刊论文

编号：

62f3c9cc-ee55-4f53-b3a7-aacb5184d743
作者：

Sun, Xiaochun(孙小淳)#^[1]Cui, Manhua(崔满华)*^[2]Zhang, Aichen(张爱臣)^[1]Tong, Lingling(佟玲玲)^[1]Wang, Kun(王堃)^[1]Li, Kai^[1];Wang, Xue(王雪)^[1]Sun, Ziqian(孙子茜)^[1]Zhang, Hongye^[1];
语种：

英文
期刊：

JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH ISSN：1756-9966 2016 年 35 卷 ; JAN 13
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关键词：

microRNA miR-548c EMT Endometrial cancer Ovarian cancer
摘要：

Background: MicroRNAs (miRNAs) are a class of small non-coding RNAs, which post-transcriptionally repress the expression of genes involved in cancer initiation and progression. Although some miRNAs that target many signaling pathways (also called universe miRNAs) are supposed to play a global role in diverse human tumors, their regulatory functions in gynecological cancers remain largely unknown. We investigated the biological role and underlying mechanism of miR-548c (one universe miRNA) in endometrial and ovarian cancer.
Methods: The effects of miR-548c overexpression on cell proliferation, migration and invasion were studied in endometrial and ovarian cancer cells. TWIST1 (Twist) was identified as a direct miR-548c target by western blot analysis and luciferase activity assay. The expression of miR-548c and Twist were examined by qRT-PCR in endometrial and ovarian cancer tissues.
Results: Here, we report that miR-548c is down-regulated in endometrial and ovarian cancer tissues when compared to normal tissues, and our meta-analysis reveal that decreased miR-548c expression correlates with poor prognosis in endometrial cancer patients. We show that in endometrial and ovarian cancer cells, ectopic expression of miR-548c significantly inhibits whereas knockdown of miR-548c dramatically induces cancer cell proliferation, migration and invasion. By using luciferase reporter assay, we demonstrate that Twist, a known oncogene in endometrial and ovarian cancers, is a direct target of miR-548c. Furthermore, the expression of Twist partially abrogates the tumor suppressive effects of miR-548c on cell migration and invasion.
Conclusion: These findings suggest that miR-548c directly downregulates Twist, and provide a novel mechanism for Twist upregulation in both endometrial and ovarian cancers. The use of miR-548c may hold therapeutic potential for the treatment of Twist-overexpressing tumors.
推荐引用方式
GB/T 7714：

Sun Xiaochun,Cui Manhua,Zhang Aichen, et al. MiR-548c impairs migration and invasion of endometrial and ovarian cancer cells via downregulation of Twist [J].JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH,2016,35.
APA：

Sun Xiaochun,Cui Manhua,Zhang Aichen,Tong Lingling,&Zhang Hongye.(2016).MiR-548c impairs migration and invasion of endometrial and ovarian cancer cells via downregulation of Twist .JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH,35.
MLA：

Sun Xiaochun, et al. "MiR-548c impairs migration and invasion of endometrial and ovarian cancer cells via downregulation of Twist" .JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH 35(2016).
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