The present study evaluated the anti-inflammatory activity of fangchinoline in rheumatoid arthritis-induced rats. Rats were grouped into sham (group I), rheumatoid arthritis (group II, control), fangchinoline (2 mu M, group III), and fangchinoline (4 mu M, group IV) groups. The serum levels of lipid peroxidation, superoxide dismutase (SOD), glutathione peroxidase (Gpx), catalase, reduced glutathione (GSH), interleukin-6 (IL-6), tumor necrosis factoralpha (TNF-alpha), matrix metalloproteinases (MMPs), prostaglandin-E2 (PGE(2)), nitric oxide (NO), zinc, ceruloplasmin, uric acid, and copper were determined. Chondrocyte cell proliferation, reactive oxygen species (ROS), and cellular levels of TNF-alpha and IL-6 were assessed. Lipid peroxidation, GSH, SOD, catalase, and Gpx levels recovered to near normal levels by fangchinoline treatment. Fangchinoline treatment significantly reduced the TNF-alpha level by 17.8% and 40.8% in groups III and IV respectively, whereas IL-6 was significantly decreased by 23.2% and 45%, respectively. Fangchinoline treatment significantly decreased the MMP-3 level by 23.1% and 65.1% in groups III and IV respectively, whereas PGE(2) was significantly decreased by 31.8% and 63.8%, respectively. Fangchinoline treatment decreased NO, uric acid, ceruloplasmin, and copper levels, whereas the zinc content was increased. Chondrocyte proliferation was significantly reduced to 73.3%, 51.3%, and 42.4% at 2 mu M, 4 mu M, and 6 mu M fangchinoline treatment respectively. Intracellular ROS, TNF-alpha, and IL-6 levels were significantly reduced in the chondrocytes. Protein expression of TNF-alpha was significantly decreased by 0.27-, 0.53-, and 0.67-fold at 2 mu M, 4 mu M, and 6 mu M fangchinoline treatment respectively. In conclusion, fangchinoline is effective as an anti-inflammatory agent in rheumatoid arthritis-induced rats.
[1]Jilin Univ, Dept Anesthesiol, China Japan Union Hosp, Changchun 130033, Jilin, Peoples R China.
[2]Jilin Univ, Dept Orthopaed, China Japan Union Hosp, Changchun 130033, Jilin, Peoples R China.
[3]Jilin Univ, Norman Bethune Coll Basic Med, Chinese Minist Educ, Dept Pathogenobiol,Key Lab Zoonosis, Changchun 130033, Jilin, Peoples R China.
[4]Jilin Univ, Dept Hepatopancreatobiliary Surg, China Japan Union Hosp, Changchun 130033, Jilin, Peoples R China.
(8.0+极速模式)