首页 / 院系成果 / 成果详情页

Inhibition effect of ATRA nanostructured lipid carriers on IL-17, ICAM-1, MIP-2, MCP-1, and Ip-10 releasing and relative signal pathways induced by zymosan. 期刊论文

编号：

742698b1-3818-4928-aab8-d52472f5f98b
作者：

Zhou, Hongyan(周鸿雁)#^[1]Gao, Xunyi^[1];Zhang, Wensong(张文松)*^[2]Zhang, Hongguang^[3];Kong, Ning^[4];
语种：

英文
期刊：

BIOMEDICAL RESEARCH-INDIA ISSN：0970-938X 2017 年 28 卷 16 期 (7041 - 7046) ; 2017
收录：
关键词：

Fungal keratitis (FK) All-trans-retinoic acid (ATRA) Nanostructured lipid carriers Cytokines Chemokines
摘要：

Fungal Keratitis (FK) is the main corneal infection that is resistant to antifugi drugs. Exploring new agent will be important for the drug resistance and new infectious diseases. All-Trans-Retinoic Acid (ATRA), a bioactive derivative of vitamin A, is immunoregulatary and anti-inflammation agent by decreasing the expression of the pro-inflammatory cytokines and increasing the expression of anti-inflammatory factors. The study was to investigate the potential role of ATRA to be a supplementary method for the therapy of FK. The aim of this study was to investigate the role of ATRA Nanostructured Lipid Carriers (NLC) in the zymosan induced cytokines (IL-17, ICAM-1, MIP-2, MCP-1 and Ip-10) by Corneal Fibroblasts (CFs) and to investigate the characters of ATRA-NCL. ATRA-NLC was prepared by the method of Emulsification process from scratch. ATRA-NCL concentration was calculated by High Performance Liquid Chromatography (HPLC) method. ATRA-NCL physical stability was observed. ATRA-NCL Cytotoxicity assay was performed by the detection of Lactate Dehydrogenase (LDH). The role of ATRA-NCL on the release of IL-17, ICAM-1, MIP-2, MCP-1, IP-10 induced by zymosan was examined by ELISA. The relative NF-KB-p65, P-ERK1, 2, P-I kappa B alpha cell signal pathway was assayed by immune blot analysis. The mean diameter of ATRA-NLC was 200 nm. ATRA-NCL physical stability study showed well at 4 degrees C without precipitation and crystallization. The NLC particles were dispersed well under the scanning electron microscope (S-4800). Scanning electron microscope image of the ATRA particles were shown. ATRA-NCL showed no cytotoxic effect on KCs. ATRA-NCL inhibited zymosan-induced IL-17, ICAM-1, MIP-2, MCP-1, Ip-10 release by CKs. ATRA-NCL inhibited NF-KB-p65, P-ERK, P-I kappa B alpha signalling pathways induced by zymosan in KCs. ATRA-NLC can inhibit the releasing of cytokines (IL-17, ICAM-1, MIP-2, MCP-1, Ip-10) induced by zymosan in CFs. It was supplementary selection for the therapy of fungal keratitis.
推荐引用方式
GB/T 7714：

Zhou Hongyan,Gao Xunyi,Zhang Wensong, et al. Inhibition effect of ATRA nanostructured lipid carriers on IL-17, ICAM-1, MIP-2, MCP-1, and Ip-10 releasing and relative signal pathways induced by zymosan. [J].BIOMEDICAL RESEARCH-INDIA,2017,28(16):7041-7046.
APA：

Zhou Hongyan,Gao Xunyi,Zhang Wensong,Zhang Hongguang,&Kong Ning.(2017).Inhibition effect of ATRA nanostructured lipid carriers on IL-17, ICAM-1, MIP-2, MCP-1, and Ip-10 releasing and relative signal pathways induced by zymosan. .BIOMEDICAL RESEARCH-INDIA,28(16):7041-7046.
MLA：

Zhou Hongyan, et al. "Inhibition effect of ATRA nanostructured lipid carriers on IL-17, ICAM-1, MIP-2, MCP-1, and Ip-10 releasing and relative signal pathways induced by zymosan." .BIOMEDICAL RESEARCH-INDIA 28,16(2017):7041-7046.
条目包含文件：

文件类型：PDF,文件大小：

正在加载全文

未找到全文

浏览次数：20  下载次数：0

分享到：

浏览次数：20

下载次数：0

打印次数：0