The current study investigated the effect of plasmid small interfering RNA (psiRNA)-mediated silencing of signal transducer and activator of transcription-3 (STAT3) on the invasion, apoptosis, and expression levels of cyclin D1, caspase-3 and B-cell lymphoma-2 (Bcl-2) in C6 glioma cells. Cell invasion was determined using a Transwell assay, while the apoptosis rate and cell cycle distribution of cells were assessed using Annexin V-FITC/PI double staining. The expression levels of cyclin D1, caspase-3 and Bcl-2 proteins were measured by western blotting. Transfection with psiRNA-STAT3 was observed to significantly decrease the number of transmembrane cells compared with the control groups (P<0.05). In addition, the proportion of cells at G0/G1 phase was increased in the psiRNA-STAT3 group compared with the controls. Western blotting indicated that psiRNA-STAT3 decreased the expression of cyclin D1, caspase-3 and Bcl-2 proteins. Taken together, psiRNA-STAT3 inhibited the migration and invasive abilities, and induced the apoptosis of C6 glioma cells, possibly through regulation of the expression of cyclin D1, caspase-3 and Bcl-2 proteins.
[1]Jilin Univ, China Japan Union Hosp, Dept Neurol, Changchun 130033, Jilin, Peoples R China.
[2]Nanjing Univ Chinese Med, Dept Biotherapeut, Coll Pharm, Nanjing 210000, Jiangsu, Peoples R China.
[3]Jilin Univ, Hosp 2, Dept Hepatobiliary Surg, Changchun 130041, Jilin, Peoples R China.
[4]Jilin Univ, China Japan Union Hosp, Dept Nephrol, 126 Xiantai St, Changchun 130033, Jilin, Peoples R China.
[5]Jilin Univ, Inst Frontier Med Sci, Biomed Engn Unit, 1163 Xinmin St, Changchun 130021, Jilin, Peoples R China.
(8.0+极速模式)