Two new baccharane-type glycosides(1 and 2), together with seven known baccharane-type glycosides (3-9) hosenkoside A, hosenkoside B, hosenkoside C, hosenkoside F, hosenkoside M, hosenkoside K and hosenkoside G were isolated from the dried seeds of Impatientis balsamina L. The new baccharane-type glycosides were identified as honsenkol A-3-O-beta-D-glucopyranosyl-26-O-beta-D-glucopyranosyl-28-O-beta-D-glucopyranosyl-O-beta-D-glucopyranosyl(1) and (3S,4R,17R,20S)-17-hydroxy-3-O-beta-D-xylopyranose(12)-beta-D-glucopyranosyl-26-O-beta-D-glucopyranosyl-28-O-beta-D-glucopyranosyl-21,24-epoxybaccharane(2), respectively, by spectroscopic methods, including NMR(1D and 2D), lR and HR-ESI-MS. Compounds 1 and 2 were evaluated for in vitro inhibitory activity against the cell lines. Compound 2 with a new baccharane skeleton had anti-hepatic fibrosis activity against A375 cells in bioassay results, which means the new compounds have potential value in antitumor applications.