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Post-Transcriptional Control of Angiotensin II Type 1 Receptor Regulates Osteosarcoma Cell Death 期刊论文

编号：

85bab174-3062-4d6a-b705-0d38d9834577
作者：

Zhao, Yue(赵岳)#^[1]Xu, Kaicheng(徐凯成)^[2]Liu, Peng(刘鹏)*^[3]
语种：

英文
期刊：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY ISSN：1015-8987 2018 年 45 卷 4 期 (1581 - 1589) ; 2018
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关键词：

Osteosarcoma (OS) Angiotensin II type 1 receptor (AGTR1) MiR-1248 Chemotherapy
摘要：

Background/Aims: MicroRNAs (miRNAs) play an essential role in the tumorigenesis of osteosarcoma (OS). However, the effects of miR-1248 on chemo-resistant potential of OS have not been studied. Here, we addressed this question. Methods: The levels of miR-1248 and apoptotic protein angiotensin II type 1 receptor (AGTR1) in OS specimens were examined by RT-qPCR and Western blotting, respectively. The relationship between miR-1248 and AGTR1 was determined by analysis of Spearman"s Rank Correlation Coefficients. The patient survival was determined with Kaplan-Meier curves. Bioinformatics analyses were done to predict microRNAs (miRNAs) that target AGTR1. The functional binding of miRNAs to AGTR1 mRNA was examined by a dual luciferase reporter assay. Cell viability was determined by an CCK-8 assay. Apoptosis was determined by a fluorescence-based apoptosis assay. Results: The levels of miR-1248 were significantly elevated while the levels of AGTR1 were significantly decreased in OS specimens than in paired adjacent normal tissue. The levels of miR-1248 were negatively correlated to the levels of AGTR1. Moreover, the patients with high miR-1248 levels had poorer survival than those with low MiR-1248 levels, and the patients with low AGTR1 levels had poorer survival than those with high AGTR1 levels. MiR-1248 inhibited protein translation of AGTR1, through binding to the 3"-UTR of the AGTR1 mRNA. The AGTR1-mediated cell apoptosis was suppressed by overexpressing miR-1248, and was augmented by depleting miR-1248. Conclusion: Increased miR-1248 expression in OS may inhibit AGTR1-mediated cancer cell death in chemotherapy. The outcome of chemotherapy may be improved by the suppression of miR-1248 in OS cells. (C) 2018 The Author(s) Published by S. Karger AG, Basel
推荐引用方式
GB/T 7714：

Zhao Yue,Xu Kaicheng,Liu Peng, et al. Post-Transcriptional Control of Angiotensin II Type 1 Receptor Regulates Osteosarcoma Cell Death [J].CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2018,45(4):1581-1589.
APA：

Zhao Yue,Xu Kaicheng,Liu Peng.(2018).Post-Transcriptional Control of Angiotensin II Type 1 Receptor Regulates Osteosarcoma Cell Death .CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,45(4):1581-1589.
MLA：

Zhao Yue, et al. "Post-Transcriptional Control of Angiotensin II Type 1 Receptor Regulates Osteosarcoma Cell Death" .CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 45,4(2018):1581-1589.
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