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MicroRNA-34a targets sirtuin 1 and leads to diabetes-induced testicular apoptotic cell death 期刊论文

编号：

86840319-c5f2-47cc-9fa7-62bd603a288e
作者：

Jiao, Dan(焦丹)#^[1]Zhang, Huan(张环)#^[2]Jiang, Ziping^[3];Huang, Wenlin^[4];Liu, Zhuo^[5];Wang, Zhaohui^[6];Wang, Yonggang(王永刚)*^[7]Wu, Hao(吴昊)*^[3,8]
语种：

英文
期刊：

JOURNAL OF MOLECULAR MEDICINE-JMM ISSN：0946-2716 2018 年 96 卷 9 期 (939 - 949) ; SEP
收录：
关键词：

Apoptosis Diabetes MiR-34a SIRT1 Testis
摘要：

Testicular apoptotic cell death (TACD) contributes to diabetes mellitus (DM)-induced male infertility. MicroRNA-34a (miR-34a) is a pro-apoptotic RNA that targets sirtuin 1 (SIRT1) which provides protection against complications of (DM). However, the specific role of miR-34a in (DM)-induced TACD is unknown. MiR-34a targets Sirt1 mRNA, resulting in apoptosis. However, whether or not SIRT1 is a major target of miR-34a in (DM)-induced TACD is unclear. The present study aimed to define the role of miR-34a/SIRT1 in (DM)-induced TACD. C57BL/6 male mice were induced to (DM) by streptozotocin, for a period of 24 weeks. The expression of miR-34a and Sirt1 as well as apoptotic cell death was determined in the testes of the non-diabetic, diabetic, and the miR-34a-specific inhibitor (miR-34a-I)-treated diabetic mice. In addition, the novel SIRT1 activator SRT2104 was delivered to the mice to determine the role of SIRT1 in DM-induced TACD. The diabetic mice developed remarkable testicular oxidative stress, endoplasmic reticulum stress, and apoptotic cell death, the effects of which were significantly and similarly attenuated by both miR-34a-I and SRT2104. Mechanistically, the DM-induced testicular elevation of miR-34a and the decrease in SIRT1 protein were markedly prevented by both miR-34a-I and SRT2104, to a similar extent. The present study demonstrates a critical role of miR-34a/SIRT1 in DM-induced TACD, providing miR-34a inhibition and SIRT1 activation as novel strategies in clinical management of DM-induced male infertility.
Key messages
MiR-34a mediates diabetes-induced TACD via inhibition of SIRT1.
The novel SIRT1 activator SRT2104 attenuates diabetes-induced TACD.
MiR-34a inhibition activates SIRT1 and prevents diabetes-induced TACD.
推荐引用方式
GB/T 7714：

Jiao Dan,Zhang Huan,Jiang Ziping, et al. MicroRNA-34a targets sirtuin 1 and leads to diabetes-induced testicular apoptotic cell death [J].JOURNAL OF MOLECULAR MEDICINE-JMM,2018,96(9):939-949.
APA：

Jiao Dan,Zhang Huan,Jiang Ziping,Huang Wenlin,&Wu Hao.(2018).MicroRNA-34a targets sirtuin 1 and leads to diabetes-induced testicular apoptotic cell death .JOURNAL OF MOLECULAR MEDICINE-JMM,96(9):939-949.
MLA：

Jiao Dan, et al. "MicroRNA-34a targets sirtuin 1 and leads to diabetes-induced testicular apoptotic cell death" .JOURNAL OF MOLECULAR MEDICINE-JMM 96,9(2018):939-949.
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