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Long Non-Coding RNA H19 Protects H9c2 Cells against Hypoxia-Induced Injury by Targeting MicroRNA-139 期刊论文

编号：

9220ba82-c719-4158-a91b-253eb6792878
作者：

Gong, LiCheng(宫立程)#^[1]Xu, HaiMing(徐海明)^[1]Guo, GongLiang(郭功亮)^[1]Zhang, Tao^[2];Shi, JingWei(时景伟)^[3]Chang, Hong(常宏)*^[1]
语种：

英文
期刊：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY ISSN：1015-8987 2017 年 44 卷 3 期 (857 - 869) ; 2017
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关键词：

Long non-coding RNA H19 Acute myocardial infarction MiR-139 Sox8 PI3K/AKT MTOR MAPK
摘要：

Background/Aims: Acute myocardial infarction (AMI) occurs when blood supply to the heart is diminished (ischemia) for long time; ischemia is primarily caused due to hypoxia. The present study evaluated the effects of long non-coding RNA H19 on hypoxic rat H9c2 cells and mouse HL-1 cells. Methods: Hypoxic injury was confirmed by measuring cell viability, migration and invasion, and apoptosis using MTT, Transwell and flow cytometry assays, respectively. H19 expression after hypoxia was estimated by qRT-PCR. We then measured the effects of non-physiologically expressed H19, knockdown of miR-139 with or without H19 silence, and abnormally expressed Sox8 on hypoxia-induced H9c2 cells. Moreover, the interacted miRNA for H19 and downstream target gene were virtually screened and verified. The involved signaling pathways and the effects of abnormally expressed H19 on contractility of HL-1 cells were explored via Western blot analysis. Results: Hypoxia induced decreases of cell viability, migration and invasion, increase of cell apoptosis and up-regulation of H19. Knockdown of H19 increased hypoxia-induced injury in H9c2 cells. H19 acted as a sponge for miR-139 and H19 knockdown aggravated hypoxia-induced injury by up-regulating miR139. Sox8 was identified as a target of miR-139, and its expression was negatively regulated by miR-139. The mechanistic studies revealed that overexpression of Sox8 might decrease hypoxia-induced cell injury by activating the PI3K/AKT/mTOR pathway and MAPK. Besides, H19 promoted contractility of HL-1 cells. Conclusion: These findings suggest that H19 alleviates hypoxia-induced myocardial cell injury by miR-139-mediated up-regulation of Sox8, along with activation of the PI3K/AKT/mTOR pathway and MAPK. (C) 2017 The Author(s) Published by S.Karger AG, Basel
推荐引用方式
GB/T 7714：

Gong Li-Cheng,Xu Hai-Ming,Guo Gong-Liang, et al. Long Non-Coding RNA H19 Protects H9c2 Cells against Hypoxia-Induced Injury by Targeting MicroRNA-139 [J].CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2017,44(3):857-869.
APA：

Gong Li-Cheng,Xu Hai-Ming,Guo Gong-Liang,Zhang Tao,&Chang Hong.(2017).Long Non-Coding RNA H19 Protects H9c2 Cells against Hypoxia-Induced Injury by Targeting MicroRNA-139 .CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,44(3):857-869.
MLA：

Gong Li-Cheng, et al. "Long Non-Coding RNA H19 Protects H9c2 Cells against Hypoxia-Induced Injury by Targeting MicroRNA-139" .CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 44,3(2017):857-869.
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