Sporothrix globosa is a type of fungus that typically infects immunocompromised patients. Its prevention continues to pose a challenge. A 70-KDa glycoprotein (Gp70) of Sporothrix has been previously reported to protect host against infection from this fungus. Here, we displayed an epitope peptide (kpvqhalltplgldr) of Gp70 on the major coat protein (pIII), and investigated its efficiency as a vaccine for preventing S. globosa infection. The recombinant phage and the heat-killed S. globosa were used to immunize mice separately. In this study, we evaluated the humoral and cellular immune responses in the mice and demonstrated that recombinant phage could induce mice to produce a stronger immune response and generate antibodies to inhibit S. globosa infection. Furthermore, immunization with recombinant phage could increase the survival rate of S. globosa infection in mice. All these results together indicated that recombinant phages displaying kpvqhalltplgldr are a potential vaccine candidate against S. globosa infection.
[1]Jilin Univ, Dermatol Dept, China Japan Union Hosp, 126Xiantai St, Changchun 130033, Jilin Province, Peoples R China.
[2]Jilin Univ, Jilin Prov Key Lab Mol & Chem Genet, Hosp 2, 218 Ziqiang St, Changchun 130041, Jilin Province, Peoples R China.
[3]Second Hosp Qinhuangdao, Dept Hang Surg, Qinhuangdao 066600, Hebei, Peoples R China.
[4]Northeast Normal Univ, Inst Cytol & Genet, Sch Life Sci, 5268 Renmin St, Changchun 130024, Jilin Province, Peoples R China.
(8.0+极速模式)