DNA methylation is an important part of epigenetics. In this study, we examined the methylation state of two CpG islands in the promoter of the p16 gene in radiation-induced thymic lymphoma samples. The mRNA and protein levels of P16 were significantly reduced in radiation-induced thymic lymphoma tissue samples. Twenty-three CpG sites of the CpG islands in the p16 promoter region were detected, and the methylation percentages of -71, -63, -239, -29, -38, -40, -23, 46 CpG sites were significantly higher in radiation-induced thymic lymphoma tissue samples than those in matched non-irradiated thymus tissue samples. This study provides new evidence for the methylation state of p16 in the radiation-induced thymic lymphoma samples, which suggests that the methylation of these CpG sites in the p16 promoter may reduce its expression in the thymic lymphoma after irradiation.
[1]Beihua Univ, Jinlin, Peoples R China.
[2]Tianjin Med Univ, Dept Toxicol, Sch Publ Hlth, Tianjin, Peoples R China.
[3]Jilin Univ, Dept Toxicol, Sch Publ Hlth, Changchun 130023, Peoples R China.
[4]Jilin Univ, Minist Hlth, Key Lab Radiobiol, Changchun 130023, Peoples R China.
[5]Tsinghua Univ, Natl Lab Med Mol Biol, Beijing 100084, Peoples R China.
[6]Jilin Univ, Coll Anim Sci, Changchun 130023, Peoples R China.
[7]Jilin Univ, China Japan Union Hosp, Dept Plast & Reconstruct Surg, Changchun 130023, Peoples R China.
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