Jolkinolide B (JB), an ent-abietane diterpenoid, isolated from the dried root of Euphorbia fischeriana, has been reported to have potent anti-tumor and anti-inflammatory activities. However, the effects of JB on acute lung injury (ALI) and underlying molecular mechanisms have not been investigated. The present study aimed to investigate the effect of JB on lipopolysaccharide (LPS)-induced ALI. Male C57BL/6 mice were pretreated with dexamethasone or JB 1 h before intranasal instillation of LPS. The results showed that JB markedly attenuated LPS-induced histological alterations, lung edema, inflammatory cell infiltration, myeloperoxidase (MPO) activity as well as the production of TNE-alpha, IL-6 and IL-1 beta. Furthermore, JB also significantly inhibited LPS-induced the degradation of I kappa B alpha. and phosphorylation of NF-kappa B p65 and MAPK. Therefore, our study provides the first line of evidence that pretreatment of JB has a protective effect on LPS-induced ALI in mice. The anti-inflammatory mechanism of JB may be attributed to its suppression of NF-kappa B and MAPK activation. (C) 2015 Elsevier B.V. All rights reserved.
[1]Jilin Univ, Emergency Dept, Changchun 130041, Peoples R China.
[2]Jilin Univ, Intens Care Unit, Changchun 130041, Peoples R China.
[3]Tumor Hosp Jilin Prov, Changchun 130022, Peoples R China.
[4]Jilin Univ, Dept Orthoped, Changchun 130041, Peoples R China.
[5]Jilin Univ, Cent Lab, Clin Hosp 2, Changchun 130041, Peoples R China.
[6]Jilin Univ, Cent Lab, Changchun 130041, Peoples R China.
[7]Jilin Univ, Med Examinat Ctr, China Japan Union Hosp, Changchun 130033, Peoples R China.
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