Salting-out homogenous extraction followed by ionic liquid/ionic liquid dispersive liquid-liquid micro extraction system was developed and applied to the extraction of sulfonamides in blood. High-performance liquid chromatography was applied to the determination of the analytes. The blood sample was centrifuged to obtain the serum. After the proteins in the serum were removed, in the presence of acetonitrile, ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate, dipotassium hydrogen phosphate, ionic "liquid 1-Hexyl-3-methylimidazolium hexafluorophosphate were added into the resulting solution. After the resulting mixture was ultrasonically shaken and centrifuged, the precipitate was separated. The acetonitrile was added in the precipitate and the analytes were extracted into the acetonitrile phase. The parameters affecting the extraction efficiency, such as volume of ionic liquid, amount of dipotassium hydrogen phosphate, volume of dispersant, extraction time and temperature were investigated. The limits of detection of sulfamethizole (STZ), sulfachlorpyridazine (SCP), sulfamethoxazole (SMX) and Sulfisoxazole (SSZ) were 4.78, 3.99, 5.21 and 3.77 mu g L-1, respectively. When the present method was applied to the analysis of real blood samples, the recoveries of analytes ranged from 90.0% to 113.0% and relative standard deviations were lower than 7.2%.
[1]Jilin Univ, China Japan Union Hosp, Xiantai St 126, Changchun 130033, Peoples R China.
[2]Jilin Univ, China Japan Union Hosp, Dept Hand Surg, Xiantai St 126, Changchun 130033, Peoples R China.
[3]Jilin Univ, Coll Pharm, Xinmin St 1163, Changchun 130033, Peoples R China.
[4]Jilin Univ, Coll Chem, Qianjin St 2699, Changchun 130012, Peoples R China.
[5]Jilin Univ, Dept Clin Med, Xinmin St 828, Changchun 130021, Peoples R China.
(8.0+极速模式)