Background: Wallerian degeneration (WD) in injured peripheral nerves is associated with a large number of up-or down-regulated genes, but the effects of these changes are poorly understood. In our previous studies, we reported some key factors that are differentially expressed to activate nerve degeneration and regeneration during WD. Here, we determined the effects of secreted phosphoprotein 1 (Spp1) on WD after rat sciatic nerve injury.
Results: Spp1 was upregulated from 6 h to 14 days after sciatic nerve injury. Altered expression of Spp1 in Schwann cells (SC) resulted in altered mRNA and protein expression levels for cytokines, c-Fos, PKCa and phospho-ERK/ERK and affected SC apoptosis in vitro. Silencing of Spp1 expression in SCs using siRNA technology reduced proliferation and promoted migration of SCs in vitro. By contrast, overexpression of Spp1 promoted proliferation and reduced migration in SCs in vitro. Differential expression of Spp1 after sciatic nerve injury in vivo altered the expression of cytokines, c-Fos, PKCa, and the p-ERK/ERK pathway.
Conclusions: Spp1 is a key regulatory factor that affects nerve degeneration and regeneration through c-Fos, PKCa and p-ERK/ERK pathways after rat sciatic nerve injury. These results shed new light on the role of Spp1 in nerve degeneration and regeneration during WD.
[1]China Japan Union Hosp Jilin Univ, 126 Xiantai Rd, Changchun 130033, Jilin, Peoples R China.
[2]Nantong Univ, CoInnovat Ctr Neuroregenerat, Sch Life Sci, Jiangsu Key Lab Neuroregenerat, 19 Qixiu Rd, Nnatong 226001, Jiangsu, Peoples R China.
[3]Affiliated Hosp Nantong Univ, 20 Xisi Rd, Nantong 226001, Jiangsu, Peoples R China.
(8.0+极速模式)