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mTOR signal pathway contribute to bone cancer pain 期刊论文

编号：

bcc65f65-af48-42aa-b0d0-29a7e75f5945
作者：

Zhang, Jian#^[1]Wang, Luping#^[2]Wang, Hushan^[1];Su, Zhenbo(苏振波)*^[3]Pang, Xiaochuan(庞晓川)*^[4]
语种：

英文
期刊：

MOLECULAR PAIN ISSN：1744-8069 2019 年 15 卷 ; FEB
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关键词：

Bone cancer mechanical hyperalgesia thermal hyperalgesia cytokines mTOR
摘要：

Background: Pain is one of the most common and distressing symptoms suffered by patients with progression of cancer; however, the mechanisms responsible for hyperalgesia are not well understood. Since the midbrain periaqueductal gray is an important component of the descending inhibitory pathway controlling on central pain transmission, in this study, we examined the role for pro-inflammatory cytokines of the periaqueductal gray in regulating mechanical and thermal hyperalgesia evoked by bone cancer via phosphatidylinositide 3-kinase (PI3K)-mammalian target of rapamycin (mTOR) signals.
Methods: Breast sarcocarcinoma Walker 256 cells were implanted into the tibia bone cavity of rats to induce mechanical and thermal hyperalgesia. Western blot analysis and ELISA were used to examine PI3K/protein kinase B (Akt)/mTOR and pro-inflammatory cytokine receptors and the levels of interleukin (IL)-1 beta, IL-6, and tumor necrosis factor-alpha (TNF-alpha).
Results: Protein expression levels of p-PI3K/p-Akt/p-mTOR were amplified in the periaqueductal gray of bone cancer rats, and blocking PI3K-mTOR pathways in the periaqueductal gray attenuated hyperalgesia responses. In addition, IL-1 beta, IL-6, and TNF-alpha were elevated in the periaqueductal gray of bone cancer rats, and expression of their respective receptors (namely, IL-1R, IL-6R, and tumor necrosis factor receptor (TNFR) subtype TNFR1) was upregulated. Inhibition of IL-1R, IL-6R, and TNFR1 alleviated mechanical and thermal hyperalgesia in bone cancer rats, accompanied with downregulated PI3K-mTOR.
Conclusions: Our data suggest that upregulation of pro-inflammatory cytokine signal in the periaqueductal gray of cancer rats amplifies PI3K-mTOR signal in this brain region and alters the descending pathways in regulating pain transmission, and this thereby contributes to the development of bone cancer-induced pain.
推荐引用方式
GB/T 7714：

Zhang Jian,Wang Luping,Wang Hushan, et al. Neuroinflammation and central PI3K/Akt/mTOR signal pathway contribute to bone cancer pain [J].MOLECULAR PAIN,2019,15.
APA：

Zhang Jian,Wang Luping,Wang Hushan,Su Zhenbo,&Pang Xiaochuan.(2019).Neuroinflammation and central PI3K/Akt/mTOR signal pathway contribute to bone cancer pain .MOLECULAR PAIN,15.
MLA：

Zhang Jian, et al. "Neuroinflammation and central PI3K/Akt/mTOR signal pathway contribute to bone cancer pain" .MOLECULAR PAIN 15(2019).
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