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Gene expression profiling of CD133-positive cells in coronary artery disease 期刊论文

编号：

ca2afd9f-5251-489e-acb6-68cb0aad692a
作者：

Li, Jiayu(李佳彧)#^[1]Zhou, Changyu(周长玉)*^[2]Li, Jiarui^[3];Wan, Yingchun(宛迎春)^[4]Li, Tao(李涛)^[5]Ma, Piyong(马丕勇)^[6]Wang, Yingjian(王瑛坚)^[7]Sang, Haiyan(桑海燕)^[1]
语种：

英文
期刊：

MOLECULAR MEDICINE REPORTS ISSN：1791-2997 2015 年 12 卷 5 期 (7512 - 7516) ; NOV
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关键词：

coronary artery disease gene expression profiles differentially expressed genes functional enrichment analysis small molecules
摘要：

Gene expression profiles of CD133-positive cells from patients with coronary artery disease (CAD) were analyzed to identify key genes associated with cardiac therapy. Furthermore, the effect of exercise on gene expression was also investigated. Gene expression data set (accession number: GSE18608) was downloaded from the Gene Expression Omnibus, including blood samples from four healthy subjects (H), and from 10 patients with coronary artery disease at baseline (B) and after 3 months (3M) of exercise. Differential analysis was performed for H vs. B and H vs. 3M using limma package of R. Two-way cluster analysis was performed using the expression levels of the differentially expressed genes (DEGs) by package pheatmap of R. Functional enrichment analysis was applied on the DEGs using the Database for Annotation, Visualization and Integrated Discovery. Relevant small molecules were predicted using the Connectivity map database (cMap). A total of 131 and 71 DEGs were identified in patients with CAD prior to and following 3 months of exercise. The two groups of DEGs were compared and 44 genes overlapped. In cluster analysis with the expression levels of the common DEGs, patients with CAD could be well separated from the healthy controls. Functional enrichment analysis showed that response to peptide hormone stimulus and anti-apoptosis pathways were significantly enriched in the common DEGs. A total of 12 relevant small molecules were revealed by cMap based upon the expression levels of common DEGs, such as 5252917 and MG-262. Three months of exercise in part normalized the gene expression in CAD patients. The genes not altered by exercise may be the targets of small molecules, such as 5252917 and MG-262.
推荐引用方式
GB/T 7714：

Li Jiayu,Zhou Changyu,Li Jiarui, et al. Gene expression profiling of CD133-positive cells in coronary artery disease [J].MOLECULAR MEDICINE REPORTS,2015,12(5):7512-7516.
APA：

Li Jiayu,Zhou Changyu,Li Jiarui,Wan Yingchun,&Sang Haiyan.(2015).Gene expression profiling of CD133-positive cells in coronary artery disease .MOLECULAR MEDICINE REPORTS,12(5):7512-7516.
MLA：

Li Jiayu, et al. "Gene expression profiling of CD133-positive cells in coronary artery disease" .MOLECULAR MEDICINE REPORTS 12,5(2015):7512-7516.
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