Objectives: To quantify microRNA-9 (miR-9) concentrations in the serum of patients with osteosarcoma; to explore its relationship with clinicopathological characteristics and prognosis of osteosarcoma.
Method: Serum miR-9 was quantified via real-time reverse transcription-polymerase chain reaction in patients with osteosarcoma and healthy control subjects. Overall survival was evaluated using the Kaplan-Meier method.
Results: Serum miR-9 was significantly upregulated in patients with osteosarcoma (n = 118) compared with healthy control subjects (n=60); its upregulation was significantly associated with advanced tumour-node-metastasis stage, larger tumour size and presence of distant metastasis. Overall survival duration was significantly shorter in patients with relatively high miR-9 concentrations compared with those with relatively low miR-9 concentrations.
Conclusions: Serum miR-9 concentrations are significantly increased in patients with osteosarcoma compared with healthy controls. Upregulation of miR-9 is associated with tumour stage, size and metastasis. Serum miR-9 quantification may represent a useful diagnostic and prognostic marker of osteosarcoma.
[1]Jilin Univ, Dept Ultrasonog, China Japan Union Hosp, Changchun 130033, Peoples R China.
[2]Jilin Univ, Dept Orthopaed, China Japan Union Hosp, Changchun 130033, Peoples R China.
(8.0+极速模式)