Objective: The inflammatory process of infectious fungus is relative to macrophages, helper T cells, neutrophils, dendritic cells, and Treg cells, and is characterized by proinflammatory, chemotactic and regulatory cytokines. Zymosan was component of the yeast cell wall which stimulated macrophages producing proinflammatory moleculars. All trans retinoid (ATRA) was well known for its inflammation suppressing effect. We sought to investigate the role of ATRA nanostructured lipid carriers (NLC) in the zymosan induced cytokines (IL-1 beta, TNF-alpha, IL-6, IL-8) and to investigate the approach of ATRA incorporated in the nanoparticles. Methods: ATRA-NLC was prepared by the method of emulsification process from scratch and the light fastness research was performed by IPA-ATRA detection. Then the particle size and concentration of ATRA-NLC was analyzed. Four to seven passages in monolayer cultured rabbit corneal fibroblasts(RCFs) were prepared for experiments. The concentrations of IL-1, TNF-alpha, IL-6, IL-8 induced by zymosan in culture supernatants were also determined with the use of ELISA kits. The ability of cells proliferation was assayed using CCK-8 according to the instructions. Results: The calculated ATRA-NLC solution concentration is 17.9 mu g/mL. The value of Peak area was detected by HPLC. ATRA-NCL showed good light resistance. ATRA-NLC average particle size was detected by the particle size analyzer. The mean diameter was 200 nm. RFCs were incubated in the absence or presence of ATRA-NLC in the additional presence of zymosan for 24 h. ATRA-NCL can inhibit the release of IL-1 beta, TNF-alpha, IL-8, IL-6 in a dose manner. Different concentrations of ATRA-NCL at different time points showed no inhibition effect on the ability of cells proliferation. ATRA-NCL at different time points showed no inhibition effect on the ability of cell viability. ATRA-NCL showed mild stimulation of cell viability. ATRA-NCL showed no effect on cell proliferation. Conclusions: ATRA-NLC can inhibit the cytokines including RFCs IL-1 beta, TNF-alpha, IL-8, IL-6 releasing induced by zymosan.
[1]Jilin Univ, China Japan Union Hosp, Dept Ophthalmol, Changchun, Jilin, Peoples R China.
[2]Jilin Univ, Hosp 2, Dept Ophthalmol, Changchun, Jilin, Peoples R China.
[3]Jilin Univ, Coll Chem, Dept Chem Engn & Applicat, Changchun, Jilin, Peoples R China.
[4]Jilin Univ, Coll Pharm, Changchun, Jilin, Peoples R China.
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