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Sappanone A prevents hypoxia-induced injury in PC-12 cells by down-regulation of miR-15a 期刊论文

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e491f31b-8e87-4898-baf1-142ea7dedfb4
状态：

撤稿成果;
作者：

Kang, Chunyang(康春阳)#^[1]Gao, Jian(高键)#^[2]Kang, Mingyang^[3];Liu, Xiaoyang(刘晓阳)^[1]Fu, Yao(付尧)^[2]Wang, Libo(王立波)*^[1]
语种：

英文
期刊：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES ISSN：0141-8130 2019 年 123 卷 (35 - 41) ; FEB 15
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关键词：

Sappanone A Cerebral ischemia Hypoxia
摘要：

Objective: We aimed to explore the effect of Sappanone A on neurologic damage induced by hypoxia.
Methods: PC-12 cells were pre-treated with Sappanone A and were simulated by hypoxia. miRNA transfection was performed to overexpress or suppress the expression of miR-15a in PC-12 cells. Cell viability, apoptosis, migration, and expression levels of miR-15a were tested to evaluate the in vitro impact of Sappanone A on hypoxiainjured PC-12 cells.
Results: Hypoxia exposure induced a significant damage in PC-12 cells, as evidenced by the repressed cell growth, the induced apoptosis and the impaired migrating capacity. Sappanone A pretreatment protected PC-12 cells against hypoxia-mediated cell damage. More interestingly, Sappanone A treatment down-regulated miR-15a, and the neuroprotective effects of Sappanone A were attenuated by miR-15a overexpression while were accelerated by miR-15a suppression. Finally, Sappanone A significantly activated Wnt/beta-catenin and PI3K/AKT signaling pathways. And the activation of these two signaling induced by Sappanone A were repressed by miR-15a over expression and were enhanced by miR-15a suppression.
Conclusion: Sappanone A exerted protective activity in PC-12 cells which were stimulated by hypoxia. One of the possible mechanisms of the neuroprotective effect is that: Sappanone A down-regulated the expression of miR-15a, and thus activated Wnt/beta-catenin and PI3M/AKT signaling pathways. (C) 2018 Published by Elsevier B.V.
推荐引用方式
GB/T 7714：

Kang Chunyang,Gao Jian,Kang Mingyang, et al. Sappanone A prevents hypoxia-induced injury in PC-12 cells by down-regulation of miR-15a [J].INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,123:35-41.
APA：

Kang Chunyang,Gao Jian,Kang Mingyang,Liu Xiaoyang,&Wang Libo.(2019).Sappanone A prevents hypoxia-induced injury in PC-12 cells by down-regulation of miR-15a .INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,123:35-41.
MLA：

Kang Chunyang, et al. "Sappanone A prevents hypoxia-induced injury in PC-12 cells by down-regulation of miR-15a" .INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 123(2019):35-41.
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