This study was designed to investigate the effects of siRNA-mediated silencing of Bmi-1 gene expression on proliferation of AGS gastric cancer cell. siRNA Bmi-1 was transfected into human AGS gastric cancer cells by liposome (as siRNA Bmi-1 group) with negative control (as control group); the expressions of Bmi-1 and apoptosis-related genes like P21, Bax, and Bcl-2 in AGS cells were determined by Western blot method; the apoptosis of AGS cells was detected by flow cytometry double staining and Hoechst staining; and cell cycle was measured by flow cytometry. Compared with the control group, the expression of Bmi-1 in the siRNA Bmi-1 group was significantly decreased (P < 0.05), the apoptosis rate was increased (P < 0.05), and cell cycles were arrested at G1 phase (P < 0.05); the expression level of P21 and Bax in cells was significantly up-regulated while that of Bcl-2 down-regulated (P < 0.05). The down regulation of Bmi-1 can inhibit the proliferation of AGS gastric cancer cell and promote its apoptosis, which takes such effects mainly by up-regulating P21 as well as Bax and down-regulating Bcl-2.
[1]Jilin Univ, Dept Nephrol, China Japan Union Hosp, Changchun, Jilin, Peoples R China.
[2]Jilin Univ, Dept Neurol, China Japan Union Hosp, Changchun, Jilin, Peoples R China.
[3]Jilin Univ, Dept Nucl Med, China Japan Union Hosp, Changchun 130012, Jilin, Peoples R China.
[4]Jilin Univ, Coll Basic Med Sci, Changchun, Jilin, Peoples R China.
(8.0+极速模式)