Apoptin is a chicken anemia virus-derived, p53-independent, bcl-2-insensitive apoptotic protein with the ability to specifically induce apoptosis of various human tumor cells, but not of normal diploid cells. To explore the application of apoptin in tumor gene therapy, we used a recombinant fowlpox virus expressing apoptin protein (vFV-Apoptin) to investigate the anti-tumor effectes of vFV-Apoptin on human cervical carcinoma(HeLa) cells in vivo and in vitro through 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide(MTT) assay, acridine orage/ethidium bromide(AO/EB) and annexin V staining test, respectively. The results show that vFV-Apoptin inhibites the proliferation of HeLa cells in vitro through inducing the apoptosis of HeLa cells, and the inhibition effect of vFV-Apoptin has a dose-effect and time-effect relationship. The results of animal models show that vFV-Apoptin significantly inhibits tumor growth, extends the lifespan of animals and improves the mean survival. Experimental results indicate that vFV-Apoptin has a potential application in the tumor gene therapy.
[1]Acad Mil Med Sci, Lab Genet Engn PLA, Changchun 130062, Peoples R China.
[2]Jilin Univ, Hosp 1, Dept Gynecol & Obstet, Changchun 130021, Peoples R China.
[3]Tumor Hosp Jilin Prov, Dept Head & Neck Surg, Changchun 130012, Peoples R China.
[4]Jilin Univ, China Japan Union Hosp, Dept Urol, Changchun 130033, Peoples R China.
[5]Jilin Univ, Hosp 1, Dept Gastroenterol, Changchun 130021, Peoples R China.
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