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Complement proteins bind to nanoparticle protein corona and undergo dynamic exchange in vivo 期刊论文 WOS高被引论文

编号：

196a2431-5559-4602-ae6c-9e6d490a1a1c
作者：

Chen, Fangfang(陈芳芳)#^[1,2]Wang, Guankui#^[2]Griffin, James I.^[2];Brenneman, Barbara^[2];Banda, Nirmal K.^[3];Holers, V. Michael^[3];Backos, Donald S.^[4];Wu, LinPing^[5];Moghimi, Seyed Moein^[5,6,7];Simberg, Dmitri*^[2]
语种：

英文
期刊：

NATURE NANOTECHNOLOGY ISSN：1748-3387 2017 年 12 卷 4 期 (387 - 393) ; APR
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摘要：

When nanoparticles are intravenously injected into the body, complement proteins deposit on the surface of nanoparticles in a process called opsonization. These proteins prime the particle for removal by immune cells and may contribute toward infusion-related adverse effects such as allergic responses. The ways complement proteins assemble on nanoparticles have remained unclear. Here, we show that dextran-coated superparamagnetic iron oxide core-shell nanoworms incubated in human serum and plasma are rapidly opsonized with the third complement component (C3) via the alternative pathway. Serum and plasma proteins bound to the nanoworms are mostly intercalated into the nanoworm shell. We show that C3 covalently binds to these absorbed proteins rather than the dextran shell and the protein-bound C3 undergoes dynamic exchange in vitro. Surface-bound proteins accelerate the assembly of the complement components of the alternative pathway on the nanoworm surface. When nanoworms pre-coated with human plasma were injected into mice, C3 and other adsorbed proteins undergo rapid loss. Our results provide important insight into dynamics of protein adsorption and complement opsonization of nanomedicines.
推荐引用方式
GB/T 7714：

Chen Fangfang,Wang Guankui,Griffin James I., et al. Complement proteins bind to nanoparticle protein corona and undergo dynamic exchange in vivo [J].NATURE NANOTECHNOLOGY,2017,12(4):387-393.
APA：

Chen Fangfang,Wang Guankui,Griffin James I.,Brenneman Barbara,&Simberg Dmitri.(2017).Complement proteins bind to nanoparticle protein corona and undergo dynamic exchange in vivo .NATURE NANOTECHNOLOGY,12(4):387-393.
MLA：

Chen Fangfang, et al. "Complement proteins bind to nanoparticle protein corona and undergo dynamic exchange in vivo" .NATURE NANOTECHNOLOGY 12,4(2017):387-393.
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